ProtSuite Results — 6cf62453526e — Bioinformatics Lab, Brooklyn College

Analysis Progress

Sequence retrieval

Fetches sequence from UniProt / NCBI or parses your FASTA

Done

Sequence properties

MW, pI, GRAVY, stability — instant local calculation

Done

BLAST homology search

blastp vs SwissProt via NCBI — typically 2–10 min

Done

Pfam domain annotation (HMMER)

hmmscan via EBI HMMER API — typically ~1 min

Done

Signal peptide & TM (Phobius)

Phobius via EBI Job Dispatcher — ~30 sec

Done

Signal peptide (SignalP)

SignalP-4.1 via EBI — independent SP cross-validation, ~30 sec

Skipped

CDD conserved domains (NCBI)

Batch Web CD-Search — typically ~1–2 min

Done

ScanProsite patterns & sites

ExPASy ScanProsite — motifs, active sites, binding sites

Done

UniProtKB feature map

Curated UniProt positional features when an accession is available

Skipped

SMART domain architecture

SMART web adapter — best-effort HTML parsing

Skipped

InterProScan (integrative)

EBI InterProScan — slowest, most comprehensive (~5–8 min)

Error

Coiled-coil prediction (Waggawagga)

Waggawagga — Marcoil/Multicoil2/Ncoils/Paircoil2 heptad-repeat regions (~10–30 sec)

Done

DisorderPred companion

Optional disorder and low-complexity view with IUPred3 / ANCHOR2 comparisons

Skipped

SSPred consensus companion

Optional secondary-structure consensus bundled into the ProtSuite report

Skipped

Merge & deduplicate annotations

Combines all sources into one clean annotation set

Done

Domain architecture figure

PROSITE MyDomains figure (or SVG fallback)

Pending

ProtSuite Service Status

BLAST UP

core

NCBI BLAST remote search

HMMER UP

core

Pfam domain scan

Phobius UP

core

Signal peptide and TM topology

CDD UP

core

NCBI conserved domains

ScanProsite UP

core

Patterns and functional sites

UniProtKB features UP

core

Curated UniProt positional features for domains, motifs, regions, and sites

Coils UP

core

Waggawagga coiled-coil prediction (Marcoil/Multicoil2/Ncoils/Paircoil2)

SMART UP

experimental

Best-effort HTML parsing

InterProScan UP

slow

Broad integrative annotation

SignalP UP

core

DTU SignalP-5.0 — independent signal peptide cross-check for Phobius

DisorderPred UP

companion

Companion disorder profile using NetSurf plus IUPred3 / ANCHOR2 comparison.

SSPred consensus UP

companion

Optional secondary-structure consensus bundle using the SSPred service adapters.

Download: JSON report Text summary Open StructMap

Summary

Length

703 aa

Mol. Weight

77,694 Da

7.98

GRAVY

0.1127

Instability

40.25 (Unstable)

BLAST hits

Domains / motifs

Functional sites

TM helices

Signal peptide

Yes

Run profile: BLAST, HMMER, Phobius, CDD, ScanProsite, UniProtKB features, Coils, InterProScan

Likely 5-15 minutes depending on remote queues.

Top BLAST Hit

Antigen peptide transporter 2

Homo sapiens

Accession
Q03519

E-value
0.00e+00

Identity
99.9%

Coverage
97.6%

Warnings

Interproscan InterProScan job iprscan5-R20260728-161616-0680-54312768-p1m ended with status: ERROR

Sequence Information

Description

NP_000535.3 antigen peptide transporter 2 isoform 1 [Homo sapiens]

Organism

Homo sapiens

Source

NCBI

Sequence (703 aa)

MRLPDLRPWTSLLLVDAALLWLLQGPLGTLLPQGLPGLWLEGTLRLGGLWGLLKLRGLLGFVGTLLLPLCLATPLTVSLRALVAGASRAPPARVASAPWSWLLVGYGAAGLSWSLWAVLSPPGAQEKEQDQVNNKVLMWRLLKLSRPDLPLLVAAFFFLVLAVLGETLIPHYSGRVIDILGGDFDPHAFASAIFFMCLFSFGSSLSAGCRGGCFTYTMSRINLRIREQLFSSLLRQDLGFFQETKTGELNSRLSSDTTLMSNWLPLNANVLLRSLVKVVGLYGFMLSISPRLTLLSLLHMPFTIAAEKVYNTRHQEVLREIQDAVARAGQVVREAVGGLQTVRSFGAEEHEVCRYKEALEQCRQLYWRRDLERALYLLVRRVLHLGVQMLMLSCGLQQMQDGELTQGSLLSFMIYQESVGSYVQTLVYIYGDMLSNVGAAEKVFSYMDRQPNLPSPGTLAPTTLQGVVKFQDVSFAYPNRPDRPVLKGLTFTLRPGEVTALVGPNGSGKSTVAALLQNLYQPTGGQVLLDEKPISQYEHCYLHSQVVSVGQEPVLFSGSVRNNIAYGLQSCEDDKVMAAAQAAHADDFIQEMEHGIYTDVGEKGSQLAAGQKQRLAIARALVRDPRVLILDEATSALDVQCEQALQDWNSRGDRTVLVIAHRLQAVQRAHQILVLQEGKLQKLAQLQEGQDLYSRLVQQRLMD

Physicochemical Properties

Length

703

MW (Da)

77694.19 Da

7.98

GRAVY

0.1127

Aromaticity

0.0797 (8.0%)

Instability Index

40.25

Helix fraction

0.3457 (34.6%)

Sheet fraction

0.3997 (40.0%)

Turn fraction

0.2603 (26.0%)

Residue Composition

17.07%

8.25%

8.11%

7.97%

6.97%

6.83%

6.4%

4.55%

4.27%

4.13%

3.41%

2.84%

2.7%

2.56%

Program
blastp

Database
SWISSPROT

Max hits
10

Returned
10

SwissProt is the curated section of UniProt — manually annotated, high-quality protein records. Hits here are reliable homologs.
Identity ≥60%: close ortholog | 30–60%: conserved family | <30%: distant or coincidental.

BLAST Hits

Show columns: Accession Protein Organism E-value Identity Coverage Score

Accession	Protein	Organism	E-value	Identity	Coverage	Bit score
Q03519	Antigen peptide transporter 2	Homo sapiens	0.0	99.9%	97.6%	3588
P36371	Antigen peptide transporter 2	Mus musculus	0.0	76.7%	99.7%	2674
P36372	Antigen peptide transporter 2	Rattus norvegicus	0.0	74.9%	99.7%	2573
Q9NP78	ABC-type oligopeptide transporter ABCB9	Homo sapiens	5.92e-164	40.8%	90.2%	1269
Q9JJ59	ABC-type oligopeptide transporter ABCB9	Mus musculus	1.30e-161	41.8%	86.1%	1253
Q9QYJ4	ABC-type oligopeptide transporter ABCB9	Rattus norvegicus	1.50e-160	41.6%	86.1%	1246
P36370	Antigen peptide transporter 1	Rattus norvegicus	1.92e-130	41.8%	76.0%	1041
Q28433	Antigen peptide transporter 1	Gorilla gorilla gorilla	1.23e-129	42.3%	76.0%	1038
Q03518	Antigen peptide transporter 1	Homo sapiens	5.03e-129	42.3%	76.0%	1033
P21958	Antigen peptide transporter 1	Mus musculus	2.73e-128	42.0%	73.4%	1027

Identity: fraction of aligned positions that are identical. Coverage: query sequence fraction covered by the best alignment. E-value: expected alignments this good by chance; <1e-5 is highly significant. Bit score: normalized alignment quality independent of database size.

Pipeline — hits per source

Source	Raw parsed	High-conf	Low-conf	Discarded	Final (deduped)
HMMER	8	7	1	0	5
Phobius	5	5	0	0	5
CDD	1	1	0	0	1
ScanProsite	3	3	0	0	3
InterProScan	0	0	0	0	0
Waggawagga	1	0	0	1	0
Total	18	17	1	0	15

Check annotations below, then click Generate Figure to build the domain architecture diagram.

Select annotations: 0 selected

E. Custom Annotations

▼ optional — write your own MyDomains commands

How to read and customize the domain architecture diagram

▼

Feature	Format	Reason
Signal peptide	`1,end,2,2,Signal peptide`	Labeled region at N-terminus
TM helix	`start,end,4,1,TM helix`	Distinct structural block
Disulfide bridge	`start,end,2`	Range marks the covalent span; no site pins
Active site	`pos,1`	Pin marker at each catalytic residue
Metal-binding	`pos,0`	Tick at individual ligand residue

A. Domain Architecture & Structural Features

Program	Interval	Name / Accession	Evidence	Confidence
CDD	1–697	3a01208 (TIGR00958)	E-value: 0.0 (-)	E: 0.0e+00
ScanProsite	153–435	ABC_TM1F 354,364,PS51216,NEBULIN 468,702,PS50893,ABC_TRANSPORTER_2 (PS50929)	PS50929	—
HMMER	153–416	PF00664	HMMER/Pfam PF00664	E: 7.2e-60
HMMER	486–635	PF00005	HMMER/Pfam PF00005	E: 7.4e-34
HMMER	490–513	PF13555	HMMER/Pfam PF13555	E: 3.2e-03
HMMER	496–686	PF13604	HMMER/Pfam PF13604	E: 2.8e-04
HMMER	498–651	PF13401	HMMER/Pfam PF13401	E: 3.3e-04
Ncoils	314–334	Coiled coil (COIL)	Ncoils 314–334	—
ScanProsite	607–621	ABC_TRANSPORTER_1 (PS00211)	PS00211	—

B. Signal Sequence & Topology

Signal Peptide

Residues 1–32

Cleavage after residue 32
Phobius

TM Helices

4 detected

56–79 99–119 149–169 189–208

C. Functional Sites

	Program	Position	Feature	Evidence	Confidence
	ScanProsite	503–510	BINDING BINDING	BINDING /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616"	—

D. Low-confidence / Supplementary

Real parsed hits below strict threshold — include in figure at your discretion

	Program	Interval	Name / Feature	Reason flagged
	HMMER	478–514	PF13481	evalue_too_high (3.54e-02 > 1e-2)

HMMER

8 hits parsed

Status	Type	Interval	Label	E-value / Score
high-conf	domain	153–416	PF00664	7.25e-60
high-conf	domain	486–635	PF00005	7.36e-34
high-conf	domain	496–686	PF13604	2.84e-04
discarded	domain	497–674	PF02463	1.59e-03
high-conf	domain	498–651	PF13401	3.28e-04
discarded	domain	498–519	PF13304	3.90e-03
low-conf	domain	478–514	PF13481	3.54e-02
high-conf	domain	490–513	PF13555	3.22e-03

Phobius

5 hits parsed

Status	Type	Interval	Label	E-value / Score
high-conf	signal_peptide	1–32	Signal peptide	—
high-conf	transmembrane	56–79	Transmembrane helix	—
high-conf	transmembrane	99–119	Transmembrane helix	—
high-conf	transmembrane	149–169	Transmembrane helix	—
high-conf	transmembrane	189–208	Transmembrane helix	—

CDD

1 hits parsed

Status	Type	Interval	Label	E-value / Score
high-conf	domain	1–697	3a01208 (TIGR00958)	0.00e+00

ScanProsite

3 hits parsed

Status	Type	Interval	Label	E-value / Score
high-conf	domain	153–435	ABC_TM1F 354,364,PS51216,NEBULIN 468,702,PS50893,ABC_TRANSPORTER_2 (PS50929)	—
high-conf	motif	607–621	ABC_TRANSPORTER_1 (PS00211)	—
high-conf	binding_site	503–510	BINDING	—

Ncoils

1 hits parsed

Status	Type	Interval	Label	E-value / Score
high-conf	coiled_coil	314–334	Coiled coil (COIL)	—

Outcome	Source	Label	Interval	Reason
low-conf	HMMER	PF13481	478–514	evalue_too_high (3.54e-02 > 1e-2)

Pfam Domain Hits (HMMER)

Raw HMMER results — merged multi-source view in Annotations tab

Domain	Start	End	Length	E-value
PF00664	153	416	264 aa	7.25e-60
PF00005	486	635	150 aa	7.36e-34
PF13604	496	686	191 aa	2.84e-04
PF02463	497	674	178 aa	1.59e-03
PF13401	498	651	154 aa	3.28e-04
PF13304	498	519	22 aa	3.90e-03
PF13481	478	514	37 aa	3.54e-02
PF13555	490	513	24 aa	3.22e-03

Signal Peptide

Phobius

✓ Detected

Residues 1–32
Cleavage after residue 32
Mature protein starts at residue 33

SignalP-4.1

Not run

Transmembrane Topology

? via Phobius (EBI)

TM Helices

Topology String

SIGNAL 1-32; 1-11 N-REGION; 12-23 H-REGION; 24-32 C-REGION; 33-55 NON CYTOPLASMIC; TRANSMEM 56-79; 80-98 CYTOPLASMIC; TRANSMEM 99-119; 120-148 NON CYTOPLASMIC; TRANSMEM 149-169; 170-188 CYTOPLASMIC; TRANSMEM 189-208; 209-703 NON CYTOPLASMIC

TM Helix Residue Ranges

#	Residue Range	Start	End	Length
1	56–79	56	79	24 aa
2	99–119	99	119	21 aa
3	149–169	149	169	21 aa
4	189–208	189	208	20 aa

{
  "_request": {
    "enabled_modules": [
      "BLAST",
      "HMMER",
      "Phobius",
      "CDD",
      "ScanProsite",
      "UniProtKB features",
      "Coils",
      "InterProScan"
    ],
    "input_type": "ncbi",
    "runtime_hint": "Likely 5-15 minutes depending on remote queues.",
    "submitted_at": "2026-07-28T15:16:14.542784+00:00",
    "submitted_value": "NP_000535.3"
  },
  "annotation_debug": {
    "deduped": 15,
    "deduped_per_source": {
      "CDD": 1,
      "HMMER": 5,
      "Ncoils": 1,
      "Phobius": 5,
      "ScanProsite": 3
    },
    "discarded": 0,
    "filter_log": [
      {
        "bucket": "low_confidence",
        "end": 514,
        "label": "PF13481",
        "reason": "evalue_too_high (3.54e-02 \u003e 1e-2)",
        "source": "HMMER",
        "start": 478
      }
    ],
    "filtered_per_source": {
      "CDD": 1,
      "HMMER": 7,
      "Ncoils": 1,
      "Phobius": 5,
      "ScanProsite": 3
    },
    "high_confidence": 17,
    "low_confidence": 1,
    "raw_per_source": {
      "CDD": 1,
      "HMMER": 8,
      "InterProScan": 0,
      "Phobius": 5,
      "ScanProsite": 3,
      "Waggawagga": 1
    },
    "raw_total": 18,
    "validated": 18
  },
  "annotation_summary": {
    "domain_count": 7,
    "has_signal_peptide": true,
    "site_count": 1,
    "source_summary": {
      "CDD": 1,
      "HMMER": 8,
      "InterProScan": 0,
      "Phobius": 5,
      "ScanProsite": 3,
      "Waggawagga": 1
    },
    "tm_helix_count": 4
  },
  "annotations": [
    {
      "accession": "",
      "description": "Signal peptide (Phobius), cleavage after residue 32",
      "display_priority": 10,
      "e_value": null,
      "end": 32,
      "evidence": "Phobius  SP 1\u201332",
      "feature_type": "signal_peptide",
      "label": "Signal peptide",
      "score": null,
      "source": "Phobius",
      "source_support": [
        "Phobius"
      ],
      "start": 1
    },
    {
      "accession": "",
      "description": "Transmembrane helix (Phobius)",
      "display_priority": 20,
      "e_value": null,
      "end": 79,
      "evidence": "Phobius  TM 56\u201379",
      "feature_type": "transmembrane",
      "label": "Transmembrane helix",
      "score": null,
      "source": "Phobius",
      "source_support": [
        "Phobius"
      ],
      "start": 56
    },
    {
      "accession": "",
      "description": "Transmembrane helix (Phobius)",
      "display_priority": 20,
      "e_value": null,
      "end": 119,
      "evidence": "Phobius  TM 99\u2013119",
      "feature_type": "transmembrane",
      "label": "Transmembrane helix",
      "score": null,
      "source": "Phobius",
      "source_support": [
        "Phobius"
      ],
      "start": 99
    },
    {
      "accession": "",
      "description": "Transmembrane helix (Phobius)",
      "display_priority": 20,
      "e_value": null,
      "end": 169,
      "evidence": "Phobius  TM 149\u2013169",
      "feature_type": "transmembrane",
      "label": "Transmembrane helix",
      "score": null,
      "source": "Phobius",
      "source_support": [
        "Phobius"
      ],
      "start": 149
    },
    {
      "accession": "",
      "description": "Transmembrane helix (Phobius)",
      "display_priority": 20,
      "e_value": null,
      "end": 208,
      "evidence": "Phobius  TM 189\u2013208",
      "feature_type": "transmembrane",
      "label": "Transmembrane helix",
      "score": null,
      "source": "Phobius",
      "source_support": [
        "Phobius"
      ],
      "start": 189
    },
    {
      "accession": "TIGR00958",
      "description": "3a01208",
      "display_priority": 30,
      "e_value": 0.0,
      "end": 697,
      "evidence": "E-value: 0.0  (-)",
      "feature_type": "domain",
      "label": "3a01208",
      "score": null,
      "source": "CDD",
      "source_support": [
        "CDD"
      ],
      "start": 1
    },
    {
      "accession": "PS50929",
      "description": "",
      "display_priority": 30,
      "e_value": null,
      "end": 435,
      "evidence": "PS50929",
      "feature_type": "domain",
      "label": "ABC_TM1F 354,364,PS51216,NEBULIN 468,702,PS50893,ABC_TRANSPORTER_2",
      "score": null,
      "source": "ScanProsite",
      "source_support": [
        "ScanProsite"
      ],
      "start": 153
    },
    {
      "accession": "PF00664",
      "description": "",
      "display_priority": 30,
      "e_value": 7.248392333845636e-60,
      "end": 416,
      "evidence": "HMMER/Pfam  PF00664",
      "feature_type": "domain",
      "label": "PF00664",
      "score": null,
      "source": "HMMER",
      "source_support": [
        "HMMER"
      ],
      "start": 153
    },
    {
      "accession": "PF00005",
      "description": "",
      "display_priority": 30,
      "e_value": 7.355528658881794e-34,
      "end": 635,
      "evidence": "HMMER/Pfam  PF00005",
      "feature_type": "domain",
      "label": "PF00005",
      "score": null,
      "source": "HMMER",
      "source_support": [
        "HMMER"
      ],
      "start": 486
    },
    {
      "accession": "PF13555",
      "description": "",
      "display_priority": 30,
      "e_value": 0.0032210942899267864,
      "end": 513,
      "evidence": "HMMER/Pfam  PF13555",
      "feature_type": "domain",
      "label": "PF13555",
      "score": null,
      "source": "HMMER",
      "source_support": [
        "HMMER"
      ],
      "start": 490
    },
    {
      "accession": "PF13604",
      "description": "",
      "display_priority": 30,
      "e_value": 0.0002835363844700845,
      "end": 686,
      "evidence": "HMMER/Pfam  PF13604",
      "feature_type": "domain",
      "label": "PF13604",
      "score": null,
      "source": "HMMER",
      "source_support": [
        "HMMER"
      ],
      "start": 496
    },
    {
      "accession": "PF13401",
      "description": "",
      "display_priority": 30,
      "e_value": 0.0003276910305401495,
      "end": 651,
      "evidence": "HMMER/Pfam  PF13401",
      "feature_type": "domain",
      "label": "PF13401",
      "score": null,
      "source": "HMMER",
      "source_support": [
        "HMMER"
      ],
      "start": 498
    },
    {
      "accession": "COIL",
      "description": "Predicted coiled-coil region (Ncoils, via Waggawagga)",
      "display_priority": 45,
      "e_value": null,
      "end": 334,
      "evidence": "Ncoils  314\u2013334",
      "feature_type": "coiled_coil",
      "label": "Coiled coil",
      "score": null,
      "source": "Ncoils",
      "source_support": [
        "Ncoils"
      ],
      "start": 314
    },
    {
      "accession": "PS00211",
      "description": "",
      "display_priority": 60,
      "e_value": null,
      "end": 621,
      "evidence": "PS00211",
      "feature_type": "motif",
      "label": "ABC_TRANSPORTER_1",
      "score": null,
      "source": "ScanProsite",
      "source_support": [
        "ScanProsite"
      ],
      "start": 607
    },
    {
      "accession": "",
      "description": "/ligand=\"ATP\" /ligand_id=\"ChEBI:CHEBI:30616\"",
      "display_priority": 75,
      "e_value": null,
      "end": 510,
      "evidence": "BINDING  /ligand=\"ATP\" /ligand_id=\"ChEBI:CHEBI:30616\"",
      "feature_type": "binding_site",
      "label": "BINDING",
      "score": null,
      "source": "ScanProsite",
      "source_support": [
        "ScanProsite"
      ],
      "start": 503
    }
  ],
  "blast": {
    "database": "swissprot",
    "hits": [
      {
        "accession": "Q03519",
        "coverage_pct": 97.6,
        "e_value": 0.0,
        "identity_pct": 99.9,
        "length": 686,
        "organism": "Homo sapiens",
        "protein_name": "Antigen peptide transporter 2",
        "query_end": 686,
        "query_start": 1,
        "score": 3588.0,
        "title": "sp|Q03519.1| RecName: Full=Antigen peptide transporter 2; Short=APT2; AltName: Full=ATP-binding cassette sub-family B me"
      },
      {
        "accession": "P36371",
        "coverage_pct": 99.7,
        "e_value": 0.0,
        "identity_pct": 76.7,
        "length": 702,
        "organism": "Mus musculus",
        "protein_name": "Antigen peptide transporter 2",
        "query_end": 701,
        "query_start": 1,
        "score": 2674.0,
        "title": "sp|P36371.1| RecName: Full=Antigen peptide transporter 2; Short=APT2; AltName: Full=ATP-binding cassette sub-family B me"
      },
      {
        "accession": "P36372",
        "coverage_pct": 99.7,
        "e_value": 0.0,
        "identity_pct": 74.9,
        "length": 703,
        "organism": "Rattus norvegicus",
        "protein_name": "Antigen peptide transporter 2",
        "query_end": 701,
        "query_start": 1,
        "score": 2573.0,
        "title": "sp|P36372.1| RecName: Full=Antigen peptide transporter 2; Short=APT2; AltName: Full=ATP-binding cassette sub-family B me"
      },
      {
        "accession": "Q9NP78",
        "coverage_pct": 90.2,
        "e_value": 5.9203e-164,
        "identity_pct": 40.8,
        "length": 766,
        "organism": "Homo sapiens",
        "protein_name": "ABC-type oligopeptide transporter ABCB9",
        "query_end": 702,
        "query_start": 69,
        "score": 1269.0,
        "title": "sp|Q9NP78.1| RecName: Full=ABC-type oligopeptide transporter ABCB9; AltName: Full=ATP-binding cassette sub-family B memb"
      },
      {
        "accession": "Q9JJ59",
        "coverage_pct": 86.1,
        "e_value": 1.3018e-161,
        "identity_pct": 41.8,
        "length": 762,
        "organism": "Mus musculus",
        "protein_name": "ABC-type oligopeptide transporter ABCB9",
        "query_end": 702,
        "query_start": 98,
        "score": 1253.0,
        "title": "sp|Q9JJ59.1| RecName: Full=ABC-type oligopeptide transporter ABCB9; AltName: Full=ATP-binding cassette sub-family B memb"
      },
      {
        "accession": "Q9QYJ4",
        "coverage_pct": 86.1,
        "e_value": 1.49798e-160,
        "identity_pct": 41.6,
        "length": 762,
        "organism": "Rattus norvegicus",
        "protein_name": "ABC-type oligopeptide transporter ABCB9",
        "query_end": 702,
        "query_start": 98,
        "score": 1246.0,
        "title": "sp|Q9QYJ4.1| RecName: Full=ABC-type oligopeptide transporter ABCB9; AltName: Full=ATP-binding cassette sub-family B memb"
      },
      {
        "accession": "P36370",
        "coverage_pct": 76.0,
        "e_value": 1.92097e-130,
        "identity_pct": 41.8,
        "length": 725,
        "organism": "Rattus norvegicus",
        "protein_name": "Antigen peptide transporter 1",
        "query_end": 698,
        "query_start": 165,
        "score": 1041.0,
        "title": "sp|P36370.2| RecName: Full=Antigen peptide transporter 1; Short=APT1; AltName: Full=ATP-binding cassette sub-family B me"
      },
      {
        "accession": "Q28433",
        "coverage_pct": 76.0,
        "e_value": 1.23022e-129,
        "identity_pct": 42.3,
        "length": 748,
        "organism": "Gorilla gorilla gorilla",
        "protein_name": "Antigen peptide transporter 1",
        "query_end": 698,
        "query_start": 165,
        "score": 1038.0,
        "title": "sp|Q28433.2| RecName: Full=Antigen peptide transporter 1; Short=APT1; AltName: Full=ATP-binding cassette sub-family B me"
      },
      {
        "accession": "Q03518",
        "coverage_pct": 76.0,
        "e_value": 5.02728e-129,
        "identity_pct": 42.3,
        "length": 748,
        "organism": "Homo sapiens",
        "protein_name": "Antigen peptide transporter 1",
        "query_end": 698,
        "query_start": 165,
        "score": 1033.0,
        "title": "sp|Q03518.3| RecName: Full=Antigen peptide transporter 1; Short=APT1; AltName: Full=ATP-binding cassette sub-family B me"
      },
      {
        "accession": "P21958",
        "coverage_pct": 73.4,
        "e_value": 2.73219e-128,
        "identity_pct": 42.0,
        "length": 724,
        "organism": "Mus musculus",
        "protein_name": "Antigen peptide transporter 1",
        "query_end": 680,
        "query_start": 165,
        "score": 1027.0,
        "title": "sp|P21958.3| RecName: Full=Antigen peptide transporter 1; Short=APT1; AltName: Full=ATP-binding cassette sub-family B me"
      }
    ],
    "max_hits_requested": 10
  },
  "companion_disorder": {},
  "companion_sspred": {},
  "completed_at": "2026-07-28 15:19 UTC",
  "figure_ok": false,
  "figure_renderer": "none",
  "hmmer": {
    "domains": [
      {
        "description": "",
        "e_value": 7.248392333845636e-60,
        "name": "PF00664",
        "seq_end": 416,
        "seq_start": 153
      },
      {
        "description": "",
        "e_value": 7.355528658881794e-34,
        "name": "PF00005",
        "seq_end": 635,
        "seq_start": 486
      },
      {
        "description": "",
        "e_value": 0.0002835363844700845,
        "name": "PF13604",
        "seq_end": 686,
        "seq_start": 496
      },
      {
        "description": "",
        "e_value": 0.0015894060788517773,
        "name": "PF02463",
        "seq_end": 674,
        "seq_start": 497
      },
      {
        "description": "",
        "e_value": 0.0003276910305401495,
        "name": "PF13401",
        "seq_end": 651,
        "seq_start": 498
      },
      {
        "description": "",
        "e_value": 0.0038964294246661537,
        "name": "PF13304",
        "seq_end": 519,
        "seq_start": 498
      },
      {
        "description": "",
        "e_value": 0.03540160833226924,
        "name": "PF13481",
        "seq_end": 514,
        "seq_start": 478
      },
      {
        "description": "",
        "e_value": 0.0032210942899267864,
        "name": "PF13555",
        "seq_end": 513,
        "seq_start": 490
      }
    ]
  },
  "job_id": "6cf62453526e",
  "low_confidence_annotations": [
    {
      "accession": "PF13481",
      "description": "",
      "display_priority": 30,
      "e_value": 0.03540160833226924,
      "end": 514,
      "evidence": "HMMER/Pfam  PF13481",
      "feature_type": "domain",
      "filter_reason": "evalue_too_high (3.54e-02 \u003e 1e-2)",
      "label": "PF13481",
      "score": null,
      "source": "HMMER",
      "source_support": [
        "HMMER"
      ],
      "start": 478
    }
  ],
  "module_errors": {
    "interproscan": "InterProScan job iprscan5-R20260728-161616-0680-54312768-p1m ended with status: ERROR"
  },
  "module_warnings": {},
  "motif_results": [],
  "phobius": {
    "has_signal_peptide": true,
    "signal_peptide_end": 32,
    "tm_count": 4,
    "tm_helices": [
      {
        "end": 79,
        "start": 56
      },
      {
        "end": 119,
        "start": 99
      },
      {
        "end": 169,
        "start": 149
      },
      {
        "end": 208,
        "start": 189
      }
    ],
    "topology": "SIGNAL 1-32; 1-11 N-REGION; 12-23 H-REGION; 24-32 C-REGION; 33-55 NON CYTOPLASMIC; TRANSMEM 56-79; 80-98 CYTOPLASMIC; TRANSMEM 99-119; 120-148 NON CYTOPLASMIC; TRANSMEM 149-169; 170-188 CYTOPLASMIC; TRANSMEM 189-208; 209-703 NON CYTOPLASMIC"
  },
  "properties": {
    "ambiguous_residues_replaced": 0,
    "amino_acid_count": {
      "A": 57,
      "C": 10,
      "D": 29,
      "E": 32,
      "F": 24,
      "G": 58,
      "H": 12,
      "I": 19,
      "K": 18,
      "L": 120,
      "M": 16,
      "N": 16,
      "P": 32,
      "Q": 49,
      "R": 45,
      "S": 48,
      "T": 30,
      "V": 56,
      "W": 12,
      "Y": 20
    },
    "amino_acid_percent": {
      "A": 8.11,
      "C": 1.42,
      "D": 4.13,
      "E": 4.55,
      "F": 3.41,
      "G": 8.25,
      "H": 1.71,
      "I": 2.7,
      "K": 2.56,
      "L": 17.07,
      "M": 2.28,
      "N": 2.28,
      "P": 4.55,
      "Q": 6.97,
      "R": 6.4,
      "S": 6.83,
      "T": 4.27,
      "V": 7.97,
      "W": 1.71,
      "Y": 2.84
    },
    "aromaticity": 0.0797,
    "gravy": 0.1127,
    "helix_fraction": 0.3457,
    "instability_index": 40.25,
    "is_stable": false,
    "isoelectric_point": 7.98,
    "length": 703,
    "molecular_weight_da": 77694.19,
    "sheet_fraction": 0.3997,
    "turn_fraction": 0.2603
  },
  "raw_annotations": [
    {
      "accession": "PF00664",
      "description": "",
      "display_priority": 30,
      "e_value": 7.248392333845636e-60,
      "end": 416,
      "evidence": "HMMER/Pfam  PF00664",
      "feature_type": "domain",
      "label": "PF00664",
      "score": null,
      "source": "HMMER",
      "source_support": [
        "HMMER"
      ],
      "start": 153
    },
    {
      "accession": "PF00005",
      "description": "",
      "display_priority": 30,
      "e_value": 7.355528658881794e-34,
      "end": 635,
      "evidence": "HMMER/Pfam  PF00005",
      "feature_type": "domain",
      "label": "PF00005",
      "score": null,
      "source": "HMMER",
      "source_support": [
        "HMMER"
      ],
      "start": 486
    },
    {
      "accession": "PF13604",
      "description": "",
      "display_priority": 30,
      "e_value": 0.0002835363844700845,
      "end": 686,
      "evidence": "HMMER/Pfam  PF13604",
      "feature_type": "domain",
      "label": "PF13604",
      "score": null,
      "source": "HMMER",
      "source_support": [
        "HMMER"
      ],
      "start": 496
    },
    {
      "accession": "PF02463",
      "description": "",
      "display_priority": 30,
      "e_value": 0.0015894060788517773,
      "end": 674,
      "evidence": "HMMER/Pfam  PF02463",
      "feature_type": "domain",
      "label": "PF02463",
      "score": null,
      "source": "HMMER",
      "source_support": [
        "HMMER"
      ],
      "start": 497
    },
    {
      "accession": "PF13401",
      "description": "",
      "display_priority": 30,
      "e_value": 0.0003276910305401495,
      "end": 651,
      "evidence": "HMMER/Pfam  PF13401",
      "feature_type": "domain",
      "label": "PF13401",
      "score": null,
      "source": "HMMER",
      "source_support": [
        "HMMER"
      ],
      "start": 498
    },
    {
      "accession": "PF13304",
      "description": "",
      "display_priority": 30,
      "e_value": 0.0038964294246661537,
      "end": 519,
      "evidence": "HMMER/Pfam  PF13304",
      "feature_type": "domain",
      "label": "PF13304",
      "score": null,
      "source": "HMMER",
      "source_support": [
        "HMMER"
      ],
      "start": 498
    },
    {
      "accession": "PF13481",
      "description": "",
      "display_priority": 30,
      "e_value": 0.03540160833226924,
      "end": 514,
      "evidence": "HMMER/Pfam  PF13481",
      "feature_type": "domain",
      "label": "PF13481",
      "score": null,
      "source": "HMMER",
      "source_support": [
        "HMMER"
      ],
      "start": 478
    },
    {
      "accession": "PF13555",
      "description": "",
      "display_priority": 30,
      "e_value": 0.0032210942899267864,
      "end": 513,
      "evidence": "HMMER/Pfam  PF13555",
      "feature_type": "domain",
      "label": "PF13555",
      "score": null,
      "source": "HMMER",
      "source_support": [
        "HMMER"
      ],
      "start": 490
    },
    {
      "accession": "",
      "description": "Signal peptide (Phobius), cleavage after residue 32",
      "display_priority": 10,
      "e_value": null,
      "end": 32,
      "evidence": "Phobius  SP 1\u201332",
      "feature_type": "signal_peptide",
      "label": "Signal peptide",
      "score": null,
      "source": "Phobius",
      "source_support": [
        "Phobius"
      ],
      "start": 1
    },
    {
      "accession": "",
      "description": "Transmembrane helix (Phobius)",
      "display_priority": 20,
      "e_value": null,
      "end": 79,
      "evidence": "Phobius  TM 56\u201379",
      "feature_type": "transmembrane",
      "label": "Transmembrane helix",
      "score": null,
      "source": "Phobius",
      "source_support": [
        "Phobius"
      ],
      "start": 56
    },
    {
      "accession": "",
      "description": "Transmembrane helix (Phobius)",
      "display_priority": 20,
      "e_value": null,
      "end": 119,
      "evidence": "Phobius  TM 99\u2013119",
      "feature_type": "transmembrane",
      "label": "Transmembrane helix",
      "score": null,
      "source": "Phobius",
      "source_support": [
        "Phobius"
      ],
      "start": 99
    },
    {
      "accession": "",
      "description": "Transmembrane helix (Phobius)",
      "display_priority": 20,
      "e_value": null,
      "end": 169,
      "evidence": "Phobius  TM 149\u2013169",
      "feature_type": "transmembrane",
      "label": "Transmembrane helix",
      "score": null,
      "source": "Phobius",
      "source_support": [
        "Phobius"
      ],
      "start": 149
    },
    {
      "accession": "",
      "description": "Transmembrane helix (Phobius)",
      "display_priority": 20,
      "e_value": null,
      "end": 208,
      "evidence": "Phobius  TM 189\u2013208",
      "feature_type": "transmembrane",
      "label": "Transmembrane helix",
      "score": null,
      "source": "Phobius",
      "source_support": [
        "Phobius"
      ],
      "start": 189
    },
    {
      "accession": "TIGR00958",
      "description": "3a01208",
      "display_priority": 60,
      "e_value": 0.0,
      "end": 697,
      "evidence": "E-value: 0.0  (-)",
      "feature_type": "domain",
      "label": "3a01208",
      "score": null,
      "source": "CDD",
      "source_support": [
        "CDD"
      ],
      "start": 1
    },
    {
      "accession": "PS50929",
      "description": "",
      "display_priority": 60,
      "e_value": null,
      "end": 435,
      "evidence": "PS50929",
      "feature_type": "domain",
      "label": "ABC_TM1F 354,364,PS51216,NEBULIN 468,702,PS50893,ABC_TRANSPORTER_2",
      "score": null,
      "source": "ScanProsite",
      "source_support": [
        "ScanProsite"
      ],
      "start": 153
    },
    {
      "accession": "PS00211",
      "description": "",
      "display_priority": 60,
      "e_value": null,
      "end": 621,
      "evidence": "PS00211",
      "feature_type": "motif",
      "label": "ABC_TRANSPORTER_1",
      "score": null,
      "source": "ScanProsite",
      "source_support": [
        "ScanProsite"
      ],
      "start": 607
    },
    {
      "accession": "",
      "description": "/ligand=\"ATP\" /ligand_id=\"ChEBI:CHEBI:30616\"",
      "display_priority": 60,
      "e_value": null,
      "end": 510,
      "evidence": "BINDING  /ligand=\"ATP\" /ligand_id=\"ChEBI:CHEBI:30616\"",
      "feature_type": "binding_site",
      "label": "BINDING",
      "score": null,
      "source": "ScanProsite",
      "source_support": [
        "ScanProsite"
      ],
      "start": 503
    },
    {
      "accession": "COIL",
      "description": "Predicted coiled-coil region (Ncoils, via Waggawagga)",
      "display_priority": 45,
      "e_value": null,
      "end": 334,
      "evidence": "Ncoils  314\u2013334",
      "feature_type": "coiled_coil",
      "label": "Coiled coil",
      "score": null,
      "source": "Ncoils",
      "source_support": [
        "Ncoils"
      ],
      "start": 314
    }
  ],
  "retrieval": {
    "error": "",
    "header": "NP_000535.3 antigen peptide transporter 2 isoform 1 [Homo sapiens]",
    "organism": "Homo sapiens",
    "sequence": "MRLPDLRPWTSLLLVDAALLWLLQGPLGTLLPQGLPGLWLEGTLRLGGLWGLLKLRGLLGFVGTLLLPLCLATPLTVSLRALVAGASRAPPARVASAPWSWLLVGYGAAGLSWSLWAVLSPPGAQEKEQDQVNNKVLMWRLLKLSRPDLPLLVAAFFFLVLAVLGETLIPHYSGRVIDILGGDFDPHAFASAIFFMCLFSFGSSLSAGCRGGCFTYTMSRINLRIREQLFSSLLRQDLGFFQETKTGELNSRLSSDTTLMSNWLPLNANVLLRSLVKVVGLYGFMLSISPRLTLLSLLHMPFTIAAEKVYNTRHQEVLREIQDAVARAGQVVREAVGGLQTVRSFGAEEHEVCRYKEALEQCRQLYWRRDLERALYLLVRRVLHLGVQMLMLSCGLQQMQDGELTQGSLLSFMIYQESVGSYVQTLVYIYGDMLSNVGAAEKVFSYMDRQPNLPSPGTLAPTTLQGVVKFQDVSFAYPNRPDRPVLKGLTFTLRPGEVTALVGPNGSGKSTVAALLQNLYQPTGGQVLLDEKPISQYEHCYLHSQVVSVGQEPVLFSGSVRNNIAYGLQSCEDDKVMAAAQAAHADDFIQEMEHGIYTDVGEKGSQLAAGQKQRLAIARALVRDPRVLILDEATSALDVQCEQALQDWNSRGDRTVLVIAHRLQAVQRAHQILVLQEGKLQKLAQLQEGQDLYSRLVQQRLMD",
    "source": "ncbi",
    "status": "ok"
  },
  "signalp": {},
  "uniprot_features": {}
}

Analysis Results