ProtSuite Service Status
BLAST
UP
core
NCBI BLAST remote search
HMMER
UP
core
Pfam domain scan
Phobius
UP
core
Signal peptide and TM topology
CDD
UP
core
NCBI conserved domains
ScanProsite
UP
core
Patterns and functional sites
UniProtKB features
UP
core
Curated UniProt positional features for domains, motifs, regions, and sites
Coils
UP
core
Waggawagga coiled-coil prediction (Marcoil/Multicoil2/Ncoils/Paircoil2)
SMART
UP
experimental
Best-effort HTML parsing
InterProScan
UP
slow
Broad integrative annotation
SignalP
UP
core
DTU SignalP-5.0 — independent signal peptide cross-check for Phobius
DisorderPred
UP
companion
Companion disorder profile using NetSurf plus IUPred3 / ANCHOR2 comparison.
SSPred consensus
UP
companion
Optional secondary-structure consensus bundle using the SSPred service adapters.
Summary
Length
1894 aa
Mol. Weight
205,555 Da
pI
9.35
GRAVY
-0.7823
Instability
59.37 (Unstable)
BLAST hits
10
Domains / motifs
12
Functional sites
0
TM helices
0
Signal peptide
Yes
Run profile: BLAST, HMMER, Phobius, CDD, ScanProsite, UniProtKB features, Coils, InterProScan, DisorderPred, SSPred consensus
Likely 5-15 minutes depending on remote queues. SSPred companion waits on multiple external predictors.
Attached Companion Analyses
DisorderPred
READY
Mean disorder 0.5767 · disordered fraction 57.0%
5 disordered region(s) · 4 low-complexity segment(s)
SSPred consensus
ERROR
Module timed out after 10 min
Top BLAST Hit
Fibronectin type III domain-containing protein 1
Homo sapiens
Warnings
Sspred_companion
Module timed out after 10 min
DisorderPred Companion
Mean disorder
0.5767
Disordered fraction
57.0%
Regions
5
Low complexity
4
Disordered regions
1–36
mean 0.98
457–1352
mean 0.9908
1416–1509
mean 0.9323
1545–1554
mean 0.5914
1566–1591
mean 0.7203
Comparison predictors
IUPred3 long
OK
Mean 0.6458 · fraction 64.7%
IUPred3 short
OK
Mean 0.5402 · fraction 60.2%
ANCHOR2
OK
Mean 0.6458 · fraction 64.7%
SSPred Consensus Companion
Module timed out after 10 min
Sequence Information
Description
NP_115921.2 fibronectin type III domain-containing protein 1 precursor [Homo sapiens]
Source
RAW
Sequence (1894 aa)
MAPEAGATLRAPRRLSWAALLLLAALLPVASSAAASVDHPLKPRHVKLLSTKMGLKVTWDPPKDATSRPVEHYNIAYGKSLKSLKYIKVNAETYSFLIEDVEPGVVYFVLLTAENHSGVSRPVYRAESPPGGEWIEIDGFPIKGPGPFNETVTEKEVPNKPLRVRVRSSDDRLSVAWKAPRLSGAKSPRRSRGFLLGYGESGRKMNYVPLTRDERTHEIKKLASESVYVVSLQSMNSQGRSQPVYRAALTKRKISEEDELDVPDDISVRVMSSQSVLVSWVDPVLEKQKKVVASRQYTVRYREKGELARWDYKQIANRRVLIENLIPDTVYEFAVRISQGERDGKWSTSVFQRTPESAPTTAPENLNVWPVNGKPTVVAASWDALPETEGKVKEYILSYAPALKPFGAKSLTYPGDTTSALVDGLQPGERYLFKIRATNRRGLGPHSKAFIVAMPTTSKADVEQNTEDNGKPEKPEPSSPSPRAPASSQHPSVPASPQGRNAKDLLLDLKNKILANGGAPRKPQLRAKKAEELDLQSTEITGEEELGSREDSPMSPSDTQDQKRTLRPPSRHGHSVVAPGRTAVRARMPALPRREGVDKPGFSLATQPRPGAPPSASASPAHHASTQGTSHRPSLPASLNDNDLVDSDEDERAVGSLHPKGAFAQPRPALSPSRQSPSSVLRDRSSVHPGAKPASPARRTPHSGAAEEDSSASAPPSRLSPPHGGSSRLLPTQPHLSSPLSKGGKDGEDAPATNSNAPSRSTMSSSVSSHLSSRTQVSEGAEASDGESHGDGDREDGGRQAEATAQTLRARPASGHFHLLRHKPFAANGRSPSRFSIGRGPRLQPSSSPQSTVPSRAHPRVPSHSDSHPKLSSGIHGDEEDEKPLPATVVNDHVPSSSRQPISRGWEDLRRSPQRGASLHRKEPIPENPKSTGADTHPQGKYSSLASKAQDVQQSTDADTEGHSPKAQPGSTDRHASPARPPAARSQQHPSVPRRMTPGRAPQQQPPPPVATSQHHPGPQSRDAGRSPSQPRLSLTQAGRPRPTSQGRSHSSSDPYTASSRGMLPTALQNQDEDAQGSYDDDSTEVEAQDVRAPAHAARAKEAAASLPKHQQVESPTGAGAGGDHRSQRGHAASPARPSRPGGPQSRARVPSRAAPGKSEPPSKRPLSSKSQQSVSAEDDEEEDAGFFKGGKEDLLSSSVPKWPSSSTPRGGKDADGSLAKEEREPAIALAPRGGSLAPVKRPLPPPPGSSPRASHVPSRLPPRSAATVSPVAGTHPWPQYTTRAPPGHFSTTPMLSLRQRMMHARFRNPLSRQPARPSYRQGYNGRPNVEGKVLPGSNGKPNGQRIINGPQGTKWVVDLDRGLVLNAEGRYLQDSHGNPLRIKLGGDGRTIVDLEGTPVVSPDGLPLFGQGRHGTPLANAQDKPILSLGGKPLVGLEVIKKTTHPPTTTMQPTTTTTPLPTTTTPRPTTATTRRTTTTRRTTTRRPTTTVRTTTRTTTTTTPTPTTPIPTCPPGTLERHDDDGNLIMSSNGIPECYAEEDEFSGLETDTAVPTEEAYVIYDEDYEFETSRPPTTTEPSTTATTPRVIPEEGAISSFPEEEFDLAGRKRFVAPYVTYLNKDPSAPCSLTDALDHFQVDSLDEIIPNDLKKSDLPPQHAPRNITVVAVEGCHSFVIVDWDKATPGDVVTGYLVYSASYEDFIRNKWSTQASSVTHLPIENLKPNTRYYFKVQAQNPHGYGPISPSVSFVTESDNPLLVVRPPGGEPIWIPFAFKHDPSYTDCHGRQYVKRTWYRKFVGVVLCNSLRYKIYLSDNLKDTFYSIGDSWGRGEDHCQFVDSHLDGRTGPQSYVEALPTIQGYYRQYRQEPVRFGNIGFGTPYYYVGWYECGVSIPGKW
Physicochemical Properties
Length
1894
MW (Da)
205555.32 Da
pI
9.35
GRAVY
-0.7823
Aromaticity
0.057 (5.7%)
Instability Index
59.37
Helix fraction
0.2587 (25.9%)
Sheet fraction
0.2809 (28.1%)
Turn fraction
0.3749 (37.5%)
Residue Composition
Program
blastpDatabase
SWISSPROTMax hits
10Returned
10
SwissProt is the curated section of UniProt — manually annotated, high-quality protein records. Hits here are reliable homologs.
Identity ≥60%: close ortholog | 30–60%: conserved family | <30%: distant or coincidental.
Identity ≥60%: close ortholog | 30–60%: conserved family | <30%: distant or coincidental.
BLAST Hits
Show columns:
| Accession | Protein | Organism | E-value | Identity | Coverage | Bit score |
|---|---|---|---|---|---|---|
| Q4ZHG4 | Fibronectin type III domain-containing protein 1 | Homo sapiens | 0.0 | 100.0% | 100.0% | 9937 |
| Q2Q0I9 | Fibronectin type III domain-containing protein 1 | Rattus norvegicus | 0.0 | 60.7% | 75.9% | 3768 |
| Q7Z7G0 | Target of Nesh-SH3 | Homo sapiens | 4.44e-85 | 52.1% | 15.9% | 786 |
| Q90610 | Neogenin | Gallus gallus | 1.68e-32 | 26.2% | 22.2% | 358 |
| Q92859 | Neogenin | Homo sapiens | 1.82e-32 | 26.1% | 22.0% | 358 |
| P43146 | Netrin receptor DCC | Homo sapiens | 2.29e-32 | 26.8% | 21.6% | 357 |
| Q63155 | Netrin receptor DCC | Rattus norvegicus | 3.73e-32 | 26.3% | 21.6% | 355 |
| P70211 | Netrin receptor DCC | Mus musculus | 5.75e-32 | 26.1% | 21.6% | 353 |
| P97798 | Neogenin | Mus musculus | 8.19e-32 | 25.8% | 22.2% | 352 |
| P97603 | Neogenin | Rattus norvegicus | 9.34e-32 | 26.1% | 22.2% | 351 |
Identity: fraction of aligned positions that are identical. Coverage: query sequence fraction covered by the best alignment. E-value: expected alignments this good by chance; <1e-5 is highly significant. Bit score: normalized alignment quality independent of database size.
Pipeline — hits per source
| Source | Raw parsed | High-conf | Low-conf | Discarded | Final (deduped) |
|---|---|---|---|---|---|
| HMMER | 18 | 10 | 5 | 3 | 9 |
| Phobius | 1 | 1 | 0 | 0 | 1 |
| CDD | 6 | 6 | 0 | 0 | 6 |
| ScanProsite | 1 | 1 | 0 | 0 | 1 |
| InterProScan | 11 | 0 | 0 | 11 | 0 |
| Waggawagga | 0 | 0 | 0 | 0 | 0 |
| Total | 37 | 29 | 5 | 3 | 13 |
Download figure
mydomains
Select annotations:
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E. Custom Annotations
▼
optional — write your own MyDomains commands
How to read and customize the domain architecture diagram
▼
A. Domain Architecture & Structural Features
?
| Program | Interval | Name / Accession | Evidence | Confidence | |
|---|---|---|---|---|---|
| HMMER | 20–83 | PF01108 | HMMER/Pfam PF01108 | E: 5.9e-03 | |
| CDD | 40–120 | FN3 (smart00060) ×5 | E-value: 3.04 × 10^-7 (-) | E: 3.0e-07 | |
| InterProScan/PANTHER | 150–1894 | TARSH-RELATED FIBRONECTIN DOMAIN-CONTAINING (PTHR23197) | PANTHER PTHR23197 E: 0.00e+00 | E: 0.0e+00 Score: 1.75e+03 |
|
| HMMER | 161–243 | PF00041 ×3 | HMMER/Pfam PF00041 | E: 3.9e-04 | |
| InterProScan/CDD | 261–354 | Fibronectin type III (IPR003961) ×4 | CDD IPR003961 E: 6.02e-10 | E: 6.0e-10 Score: 56 |
|
| InterProScan/CDD | 362–452 | Fibronectin type III (IPR003961) ×4 | CDD IPR003961 E: 1.77e-12 | E: 1.8e-12 Score: 63.3 |
|
| HMMER | 363–440 | PF16656 | HMMER/Pfam PF16656 | E: 3.3e-05 | |
| CDD | 632–1064 | PHA03307 superfamily (cl33723) | E-value: 1.11 × 10^-11 (C) | E: 1.1e-11 | |
| CDD | 811–1352 | PHA03247 superfamily (cl33720) | E-value: 1.81 × 10^-9 (N) | E: 1.8e-09 | |
| InterProScan/CDD | 1658–1749 | Fibronectin type III (IPR003961) ×4 | CDD IPR003961 E: 9.58e-13 | E: 9.6e-13 Score: 64.1 |
|
| HMMER | 1693–1749 | PF16656 | HMMER/Pfam PF16656 | E: 2.9e-04 | |
| HMMER | 1767–1891 | PF21731 | HMMER/Pfam PF21731 | E: 2.5e-50 |
B. Signal Sequence & Topology
Signal Peptide
Residues 1–34
Cleavage after residue 34
Phobius
Phobius
C. Functional Sites
?
| Program | Position | Feature | Evidence | Confidence | |
|---|---|---|---|---|---|
| None detected. (Run ScanProsite or InterProScan to find ACT_SITE, DISULFID, BINDING residues.) | |||||
D. Low-confidence / Supplementary
Real parsed hits below strict threshold — include in figure at your discretion
| Program | Interval | Name / Feature | Reason flagged | |
|---|---|---|---|---|
| HMMER | 69–124 | PF25059 | evalue_too_high (5.28e-02 > 1e-2) | |
| HMMER | 273–343 | PF09294 | evalue_too_high (2.78e-01 > 1e-2) | |
| HMMER | 1672–1734 | PF21605 | evalue_too_high (1.47e-01 > 1e-2) | |
| HMMER | 1692–1735 | PF09294 | evalue_too_high (6.16e-01 > 1e-2) | |
| HMMER | 1696–1738 | PF25059 | evalue_too_high (4.81e-01 > 1e-2) |
Pfam Domain Hits (HMMER)
?
| Domain | Description | Start | End | Length | E-value |
|---|---|---|---|---|---|
| PF21731 | 1767 | 1891 | 125 aa | 2.48e-50 | |
| PF00041 | 44 | 121 | 78 aa | 9.25e-05 | |
| PF00041 | 161 | 243 | 83 aa | 3.87e-04 | |
| PF00041 | 262 | 351 | 90 aa | 3.75e-08 | |
| PF00041 | 362 | 449 | 88 aa | 1.10e-10 | |
| PF00041 | 1658 | 1747 | 90 aa | 1.11e-09 | |
| PF16656 | 265 | 349 | 85 aa | 1.14e-03 | |
| PF16656 | 363 | 440 | 78 aa | 3.31e-05 | |
| PF16656 | 1693 | 1749 | 57 aa | 2.94e-04 | |
| PF25059 | 69 | 124 | 56 aa | 5.28e-02 | |
| PF25059 | 296 | 336 | 41 aa | 4.18e+00 | |
| PF25059 | 414 | 447 | 34 aa | 2.00e+00 | |
| PF25059 | 1696 | 1738 | 43 aa | 4.81e-01 | |
| PF09294 | 273 | 343 | 71 aa | 2.78e-01 | |
| PF09294 | 1692 | 1735 | 44 aa | 6.16e-01 | |
| PF21605 | 293 | 341 | 49 aa | 2.49e+00 | |
| PF21605 | 1672 | 1734 | 63 aa | 1.47e-01 | |
| PF01108 | 20 | 83 | 64 aa | 5.89e-03 |
Signal Peptide
?
Phobius
✓ Detected
Residues 1–34
Cleavage after residue 34
Mature protein starts at residue 35
Cleavage after residue 34
Mature protein starts at residue 35
SignalP-4.1
Not run
Transmembrane Topology
?
via Phobius (EBI)
TM Helices
0
Topology String
SIGNAL 1-34; 1-14 N-REGION; 15-26 H-REGION; 27-34 C-REGION; 35-1894 NON CYTOPLASMIC
No transmembrane helices detected.