ProtSuite Service Status
BLAST
UP
core
NCBI BLAST remote search
HMMER
UP
core
Pfam domain scan
Phobius
UP
core
Signal peptide and TM topology
CDD
UP
core
NCBI conserved domains
ScanProsite
UP
core
Patterns and functional sites
UniProtKB features
UP
core
Curated UniProt positional features for domains, motifs, regions, and sites
Coils
UP
core
Waggawagga coiled-coil prediction (Marcoil/Multicoil2/Ncoils/Paircoil2)
SMART
UP
experimental
Best-effort HTML parsing
InterProScan
UP
slow
Broad integrative annotation
SignalP
UP
core
DTU SignalP-5.0 — independent signal peptide cross-check for Phobius
DisorderPred
UP
companion
Companion disorder profile using NetSurf plus IUPred3 / ANCHOR2 comparison.
SSPred consensus
UP
companion
Optional secondary-structure consensus bundle using the SSPred service adapters.
Summary
Length
681 aa
Mol. Weight
74,354 Da
pI
4.79
GRAVY
-0.3244
Instability
26.8 (Stable)
BLAST hits
0
Domains / motifs
13
Functional sites
0
TM helices
1
Signal peptide
No
Run profile: BLAST, HMMER, Phobius, CDD, ScanProsite, UniProtKB features, Coils, InterProScan, DisorderPred, SSPred consensus
Likely 5-15 minutes depending on remote queues. SSPred companion waits on multiple external predictors.
Attached Companion Analyses
DisorderPred
READY
Mean disorder 0.1135 · disordered fraction 10.9%
2 disordered region(s) · 1 low-complexity segment(s)
SSPred consensus
ERROR
Module timed out after 10 min
Warnings
Blast
BLAST timed out after 10 minutes. Try a smaller sequence or a faster database (SwissProt).
Sspred_companion
Module timed out after 10 min
DisorderPred Companion
Mean disorder
0.1135
Disordered fraction
10.9%
Regions
2
Low complexity
1
Disordered regions
1–46
mean 0.9424
654–681
mean 0.9604
Comparison predictors
IUPred3 long
OK
Mean 0.315 · fraction 10.9%
IUPred3 short
OK
Mean 0.2688 · fraction 5.3%
ANCHOR2
OK
Mean 0.315 · fraction 10.9%
SSPred Consensus Companion
Module timed out after 10 min
Sequence Information
Description
User-submitted sequence
Source
RAW
Sequence (681 aa)
MFFNRLSAGKLLVPLSVVLYALFVVILPLQNSFHSSNVLVRGADDVENYGTVIGIDLGTTYSCVAVMKNGKTEILANEQGNRITPSYVAFTDDERLIGDAAKNQVAANPQNTIFDIKRLIGLKYNDRSVQKDIKHLPFNVVNKDGKPAVEVSVKGEKKVFTPEEISGMILGKMKQIAEDYLGTKVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVLRIVNEPTAAAIAYGLDKSDKEHQIIVYDLGGGTFDVSLLSIENGVFEVQATSGDTHLGGEDFDYKIVRQLIKAFKKKHGIDVSDNNKALAKLKREAEKAKRALSSQMSTRIEIDSFVDGIDLSETLTRAKFEELNLDLFKKTLKPVEKVLQDSGLEKKDVDDIVLVGGSTRIPKVQQLLESYFDGKKASKGINPDEAVAYGAAVQAGVLSGEEGVEDIVLLDVNALTGIETTGGVMTPLIKRNTAIPTKKSQIFSTAVDNQPTVMIKVYEGERAMSKDNNLLGKFELTGIPPAPRGVPQIEVTFALDANGILKVSATDKGTGKSESITITNDKGRLTQEEIDRMVEEAEKFASEDASIKAKVESRNKLENYAHSLKNQVNGDLGEKLEEEDKETLLDAANDVLEWLDDNFETAIAEDFDEKFESLSKVAYPITSKLYGGADGSGAADYDDEDEDDDGDYFEHDEL
Physicochemical Properties
Length
681
MW (Da)
74353.88 Da
pI
4.79
GRAVY
-0.3244
Aromaticity
0.0646 (6.5%)
Instability Index
26.8
Helix fraction
0.3554 (35.5%)
Sheet fraction
0.3598 (36.0%)
Turn fraction
0.301 (30.1%)
Residue Composition
Program
blastpDatabase
SWISSPROTMax hits
10Returned
0
SwissProt is the curated section of UniProt — manually annotated, high-quality protein records. Hits here are reliable homologs.
Identity ≥60%: close ortholog | 30–60%: conserved family | <30%: distant or coincidental.
Identity ≥60%: close ortholog | 30–60%: conserved family | <30%: distant or coincidental.
BLAST — Failed
BLAST search failed
BLAST timed out after 10 minutes. Try a smaller sequence or a faster database (SwissProt).
BLAST submits to NCBI's remote API. This can fail if NCBI servers are temporarily overloaded or the query times out (queries can take 2–10 minutes for longer sequences). Try resubmitting — NCBI BLAST queues are usually responsive within a few minutes.
Pipeline — hits per source
| Source | Raw parsed | High-conf | Low-conf | Discarded | Final (deduped) |
|---|---|---|---|---|---|
| HMMER | 7 | 2 | 1 | 4 | 2 |
| Phobius | 1 | 1 | 0 | 0 | 1 |
| CDD | 1 | 1 | 0 | 0 | 1 |
| ScanProsite | 2 | 2 | 0 | 0 | 2 |
| InterProScan | 6 | 0 | 0 | 6 | 0 |
| Waggawagga | 4 | 0 | 0 | 4 | 0 |
| Total | 21 | 16 | 1 | 4 | 14 |
Check annotations below, then click Generate Figure to build the domain architecture diagram.
Select annotations:
0 selected
E. Custom Annotations
▼
optional — write your own MyDomains commands
How to read and customize the domain architecture diagram
▼
A. Domain Architecture & Structural Features
?
| Program | Interval | Name / Accession | Evidence | Confidence | |
|---|---|---|---|---|---|
| InterProScan/NCBIFAM | 50–677 | PRK00290.1 (NF001413) | NCBIFAM NF001413 E: 1.20e-234 | E: 1.2e-234 Score: 792 |
|
| InterProScan/CDD | 50–425 | Endoplasmic reticulum chaperone BIP, nucleotide-binding doma (IPR042050) | CDD IPR042050 Score: 794.1 | Score: 794 | |
| CDD | 52–653 | HSP70 (pfam00012) ×2 | E-value: 0.0 (-) | E: 0.0e+00 | |
| HMMER | 173–419 | PF06723 | HMMER/Pfam PF06723 | E: 2.5e-17 | |
| ScanProsite | 219–282 | FILAMIN_REPEAT (PS50194) | PS50194 | — | |
| InterProScan/PANTHER | 52–610 | Heat shock protein 70 family (IPR013126) | PANTHER IPR013126 E: 4.40e-241 | E: 4.4e-241 Score: 813 |
|
| Ncoils | 291–325 | Coiled coil (COIL) | Ncoils 291–325 | — | |
| Ncoils | 550–570 | Coiled coil (COIL) | Ncoils 550–570 | — | |
| Ncoils | 574–594 | Coiled coil (COIL) | Ncoils 574–594 | — | |
| Ncoils | 612–632 | Coiled coil (COIL) | Ncoils 612–632 | — | |
| ScanProsite | 55–62 | HSP70_1 242,255,PS00329,HSP70_2 379,393,PS01036,HSP70_3 678,681,PS00014,ER_TARGE (PS00297) ×2 | PS00297 | — | |
| InterProScan/PROSITE_PATTERNS | 242–255 | Heat shock protein 70, conserved site (IPR018181) | PROSITE_PATTERNS IPR018181 | — | |
| InterProScan/PROSITE_PATTERNS | 379–393 | Heat shock protein 70, conserved site (IPR018181) | PROSITE_PATTERNS IPR018181 | — |
B. Signal Sequence & Topology
TM Helices
1 detected
C. Functional Sites
?
| Program | Position | Feature | Evidence | Confidence | |
|---|---|---|---|---|---|
| None detected. (Run ScanProsite or InterProScan to find ACT_SITE, DISULFID, BINDING residues.) | |||||
D. Low-confidence / Supplementary
Real parsed hits below strict threshold — include in figure at your discretion
| Program | Interval | Name / Feature | Reason flagged | |
|---|---|---|---|---|
| HMMER | 217–264 | PF01968 | evalue_too_high (8.17e-02 > 1e-2) |
Pfam Domain Hits (HMMER)
?
Signal Peptide
?
Phobius
Not detected
SignalP-4.1
Not run
Transmembrane Topology
?
via Phobius (EBI)
TM Helices
1
Topology String
1-11 CYTOPLASMIC; TRANSMEM 12-29; 30-681 NON CYTOPLASMIC
TM Helix Residue Ranges
| # | Residue Range | Start | End | Length |
|---|---|---|---|---|
| 1 | 12–29 | 12 | 29 | 18 aa |