ProtSuite Service Status
BLAST
UP
core
NCBI BLAST remote search
HMMER
UP
core
Pfam domain scan
Phobius
UP
core
Signal peptide and TM topology
CDD
UP
core
NCBI conserved domains
ScanProsite
UP
core
Patterns and functional sites
UniProtKB features
UP
core
Curated UniProt positional features for domains, motifs, regions, and sites
Coils
UP
core
Waggawagga coiled-coil prediction (Marcoil/Multicoil2/Ncoils/Paircoil2)
SMART
UP
experimental
Best-effort HTML parsing
InterProScan
UP
slow
Broad integrative annotation
SignalP
UP
core
DTU SignalP-5.0 — independent signal peptide cross-check for Phobius
DisorderPred
UP
companion
Companion disorder profile using NetSurf plus IUPred3 / ANCHOR2 comparison.
SSPred consensus
UP
companion
Optional secondary-structure consensus bundle using the SSPred service adapters.
Summary
Length
127 aa
Mol. Weight
13,045 Da
pI
4.6
GRAVY
-0.3189
Instability
58.76 (Unstable)
BLAST hits
5
Domains / motifs
1
Functional sites
0
TM helices
0
Signal peptide
No
Run profile: BLAST, HMMER, Phobius, CDD, ScanProsite, UniProtKB features, Coils, SMART, InterProScan, SignalP, DisorderPred, SSPred consensus
Likely 5-15 minutes depending on remote queues. SSPred companion waits on multiple external predictors.
Attached Companion Analyses
DisorderPred
READY
Mean disorder 0.8689 · disordered fraction 96.1%
2 disordered region(s) · 0 low-complexity segment(s)
SSPred consensus
READY
5 contributing predictors · mode majority vote
H 11.8% · E 0.0% · C 88.2%
Top BLAST Hit
Histone acetyltransferase p300
Homo sapiens
DisorderPred Companion
Mean disorder
0.8689
Disordered fraction
96.1%
Regions
2
Low complexity
0
Disordered regions
1–34
mean 0.8538
41–127
mean 0.905
Comparison predictors
IUPred3 long
OK
Mean 0.6536 · fraction 91.3%
IUPred3 short
OK
Mean 0.5487 · fraction 58.3%
ANCHOR2
OK
Mean 0.6536 · fraction 91.3%
SSPred Consensus Companion
Contributors
5
Helix
11.8%
Strand
0.0%
Coil
88.2%
Consensus prediction
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
Consensus support
9777779999999999999999999999999999955557999955599999999999999999999997755555555555555799999999999999999999999999997777799999999
Contributing services
| Service | Status | Prediction |
|---|---|---|
| JPred | Complete | CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC… |
| PSI | Complete | CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC… |
| Sable | Complete | CHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC… |
| SSPro | Error | Unexpected companion error: HTTPConnectionPool(host='scratch.proteomics.ics.uci.edu', port=80): Max retries exceeded with url: /cgi-bin/new_server/sql_predict.cgi (Caused by ConnectTimeoutError(<urllib3.connection.HTTPConnection object at 0x7698335dbce0>, 'Connection to scratch.proteomics.ics.uci.edu timed out. (connect timeout=None)')) |
| Yaspin | Complete | CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC… |
| Predator | Error | Server returned HTTP 503 |
| NetSurf | Complete | CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC… |
Sequence Information
Description
P300_1-127
Source
RAW
Sequence (127 aa)
MAENVVEPGPPSAKRPKLSSPALSASASDGTDFGSLFDLEHDLPDELINSTELGLTNGGDINQLQTSLGMVQDAASKHKQLSELLRSGSSPNLNMGVGGPGQVMASQAQQSSPGLGLINSMVKSPMT
Physicochemical Properties
Length
127
MW (Da)
13045.44 Da
pI
4.6
GRAVY
-0.3189
Aromaticity
0.0157 (1.6%)
Instability Index
58.76
Helix fraction
0.3307 (33.1%)
Sheet fraction
0.252 (25.2%)
Turn fraction
0.4488 (44.9%)
Residue Composition
Program
blastpDatabase
SWISSPROTMax hits
10Returned
5
SwissProt is the curated section of UniProt — manually annotated, high-quality protein records. Hits here are reliable homologs.
Identity ≥60%: close ortholog | 30–60%: conserved family | <30%: distant or coincidental.
Identity ≥60%: close ortholog | 30–60%: conserved family | <30%: distant or coincidental.
BLAST Hits
Show columns:
| Accession | Protein | Organism | E-value | Identity | Coverage | Bit score |
|---|---|---|---|---|---|---|
| Q09472 | Histone acetyltransferase p300 | Homo sapiens | 6.24e-78 | 100.0% | 100.0% | 645 |
| B2RWS6 | Histone acetyltransferase p300 | Mus musculus | 6.02e-74 | 96.0% | 99.2% | 616 |
| Q92793 | CREB-binding protein | Homo sapiens | 1.59e-25 | 61.6% | 77.2% | 256 |
| P45481 | Histone lysine acetyltransferase CREBBP | Mus musculus | 2.03e-25 | 60.2% | 77.2% | 256 |
| Q6JHU9 | Histone lysine acetyltransferase CREBBP | Rattus norvegicus | 1.56e-24 | 59.2% | 77.2% | 249 |
Identity: fraction of aligned positions that are identical. Coverage: query sequence fraction covered by the best alignment. E-value: expected alignments this good by chance; <1e-5 is highly significant. Bit score: normalized alignment quality independent of database size.
Pipeline — hits per source
| Source | Raw parsed | High-conf | Low-conf | Discarded | Final (deduped) |
|---|---|---|---|---|---|
| HMMER | 0 | 0 | 0 | 0 | 0 |
| Phobius | 0 | 0 | 0 | 0 | 0 |
| SignalP | 0 | 0 | 0 | 0 | 0 |
| CDD | 0 | 0 | 0 | 0 | 0 |
| ScanProsite | 0 | 0 | 0 | 0 | 0 |
| SMART | 3 | 1 | 0 | 2 | 1 |
| InterProScan | 0 | 0 | 0 | 0 | 0 |
| Waggawagga | 0 | 0 | 0 | 0 | 0 |
| Total | 3 | 1 | 0 | 2 | 1 |
Check annotations below, then click Generate Figure to build the domain architecture diagram.
Select annotations:
0 selected
E. Custom Annotations
▼
optional — write your own MyDomains commands
How to read and customize the domain architecture diagram
▼
A. Domain Architecture & Structural Features
?
| Program | Interval | Name / Accession | Evidence | Confidence | |
|---|---|---|---|---|---|
| SMART | 18–28 | low complexity low-complexity | SMART low complexity | — |
B. Signal Sequence & Topology
No signal peptide or transmembrane helices detected.
C. Functional Sites
?
| Program | Position | Feature | Evidence | Confidence | |
|---|---|---|---|---|---|
| None detected. (Run ScanProsite or InterProScan to find ACT_SITE, DISULFID, BINDING residues.) | |||||
Pfam domain annotation was not run for this job.
Signal Peptide
?
Phobius
Not detected
SignalP-4.1
Not detected
✓ Both predictors agree: no signal peptide.
Transmembrane Topology
?
via Phobius (EBI)
TM Helices
0
Topology String
1-127 NON CYTOPLASMIC
No transmembrane helices detected.
Motif Search Results
Hits use 1-based residue numbering. A match shows that the pattern is present — it does not prove that the associated function is active in this protein.
HExxH
H-E-x(2)-H
No hits
No matches found in this sequence.
HExxH…E (neprilysin-type)
H-E-x(2)-H-x(20,80)-E
No hits
No matches found in this sequence.
CxxC
C-x(2)-C
No hits
No matches found in this sequence.
N-x-S/T (N-glycosylation)
N-x(1)-[ST]
1 hit
| # | Start | End | Length | Matched sequence |
|---|---|---|---|---|
| 1 | 49 | 51 | 3 | NST |
R…S…E
R-x(0,200)-S-x(0,200)-E
1 hit
| # | Start | End | Length | Matched sequence |
|---|---|---|---|---|
| 1 | 15 | 83 | 69 | RPKLSSPALSASASDGTDFGSLFDLEHDLPDELINSTELGLTNGGDINQLQTSLGMVQDAASKHKQLSE |
KDEL / HDEL (ER retention)
[KH]-D-E-L
No hits
No matches found in this sequence.
Coactivator Motif
LxxLL
1 hit
| # | Start | End | Length | Matched sequence |
|---|---|---|---|---|
| 1 | 81 | 85 | 5 | LSELL |
Sequence with highlighted motif locations
1
MAENVVEPGPPSAKRPKLSSPALSASASDGTDFGSLFDLEHDLPDELINSTELGLTNGGD
61
INQLQTSLGMVQDAASKHKQLSELLRSGSSPNLNMGVGGPGQVMASQAQQSSPGLGLINS
121
MVKSPMT